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Melanin is a protein that requires tyrosine (and iron as a cofactor) from our diet (phenylalanine is the essential amino acid from which we make tyrosine)

Biologia Plantarum

Performance characteristics Synonyms ALT Sample type Serum, plasma, animal tissue Sensitivity 0.01 U/L Detection range 0.01-0.83 U/L Detection method Fluorescence method Assay type Enzyme Activity Assay time 75 min Precision Average inter-assay CV: 9.800000000000001%Average intra-assay CV: 2.3% Other instruments required Pipettor, Vortex mixer, Centrifuge Storage -20 C Valid period 6 months Dilution of sample It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.01-0.83 U/L)

Johnson B , Haymaker C, Morris VK, Dasari A, Higbie VS, Shen JP, Parseghian C, Morelli MP, Huey R, Lee MS, Willis J, Raghav KP, Yuan Y, Zebala J, DePinho RA, Kopetz S , Overman M

PMID: 38011703