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2.Lentivirus packaging (1) Take 4 L of lentivirus packaging helper plasmid mixture into a 2 mL centrifuge tube, add 4 g of lentiviral vector plasmid containing the target gene, mix gently, add 40 L of MCE Lentivirus Transfection Reagent and mix gently, incubate for 3 min at room temperature

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Various cytochemical and immunohistochemical parameters, including oligodendroglial death, gliosis, oxidative stress and demyelination, were investigated to determine the extent to which powdered and pelleted cuprizone differ in their capacity to induce the hallmarks of cuprizone-intoxication in the CNS

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