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doi: 10.1186/s12986-016-0132-8

Naturally produced in the body, it is made up of three key amino acids: cysteine, glutamine, and glycine

Thioflavin T assay Thioflavin T (ThT) assays were performed in triplicates in 96 medium binding well microplates (Greiner Bio-One, Frickenhausen, Germany) with a FP-8500 fluorescence spectrometer (Jasco, Gross-Umstadt, Germany) equipped with an FMP-825 fluorescence microplate reader (Jasco, Gross-Umstadt, Germany)

1b), whose active Fe III center is located in the center of heme (Fe-protoporphyrin IX (Fe-PpIX)), while the axially-ligated tyrosine residue (Tyr 354) can regulate the metal-centered redox potential of heme favoring hydrogen peroxide (H 2 O 2 ) decomposition 2