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Then cells were blocked with 1% bovine serum albumin (BSA) in PBS for 30 min, followed by incubation with appropriate primary antibodies at 4 C overnight and the secondary antibody for 1 h at room temperature

University of Chicago Press, Chicago, IL

Similar content being viewed by others Introduction The biogenic amine neurotransmitters including dopamine (DA), norepinephrine (NE) and serotonin (5-HT) are well-known to play important roles in regulation of mood and in neuropsychiatric disorders including addiction, depression, ADHD and schizophrenia

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What to watch for: Digestive changes: Occasionally, users might experience mild bloating or cramping when starting a new supplement