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Desired Dose: 50mcg Our Concentration: 500mcg/mL Calculation: (Desired Dose) / (Concentration) = Volume in mL So: 50mcg / 500mcg/mL = 0.1mL Now convert that 0.1mL into units on your insulin syringe: 0.1mL * 100 IU/mL = 10 IU

[PMC free article: PMC3461919] [PubMed: 23006326] 20

Silica gel (200300 mesh) was used for column chromatography

Mass Spectrometry Identity Confirmation Identity is verified on every lot by LC-MS, matching the observed mass against the theoretical 711.85 g/mol value for CHNO