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Description

Further analysis by Western blot showed that rhGPx3 mutant with excess amino acid could be recognized by the monoclonal antibody against the hexahistidine tag, but not in the case of the protein treated with factor Xa (Figure 1a and 1b)

J Pineal Res 59: 4759, 2015

In vitro experiments To plate cells for infection, aliquots of BMDMs were thawed on ice, diluted into 9 ml of DMEM, centrifuged in an Eppendorf 5810 R at 1,200 RPM (290 x G) for 5 min, and the pellet was resuspended in 10 ml BMDM media without antibiotics