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GPX2 gene deletion in hPSCs Three different sgRNAs targeting the GPX2 gene were designed using Benchling software and produced in-house, according to the protocol previously published 18

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The study focused on functional endpoints (adhesion molecules, Annexin V, oxidative stress, mitochondrial polarization), for which mean fluorescence intensity (MFI) was the appropriate metric

These devices use a vibrating mesh or a vibrating horn

In the immunohistochemistry process, tissue sections underwent dewaxing with xylene and a gradient ethanol series