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Another animal experimental study discovered that chronic treatment with berberine significantly and dose-dependently decreased levels of oxidative stress markers (MDA, GSH), thereby ameliorating cognitive deficits and cholinergic dysfunction in diabetic rats (58)

Arch Environ Contam Toxicol 23:453456

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Then, cells were washed in PBS-BSA 0.5% with 0.02% sodium azide, centrifuged at 1,500 rpm for 5 min at 4 C, counted again, and separated as 2 10 5 cells per tube