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Sticking to dietary sources or carefully dosed supplements is safest

Thus, several alternative approaches have been attempted

FAD absorption spectroscopy To assess the presence of protein-bound FAD, 100 g of recombinant WT or D285N mutant FSP1 protein or 10 g of free FAD control was resuspended in a total volume of 200 l in 50 mM sodium phosphate pH 7.4

Dermatology Research and Practice

Escherichia coli strains NEB 5-alpha, BL21(DE3), BL21 Star (DE3) (New England Biolabs, Frankfurt, Germany) or M15 (Qiagen, Hilden, Germany) were used as hosts for propagation of respective vectors and for production of recombinant His-tagged proteins were grown at 37 C in yeast tryptone (YT) broth containing 50 g/ml ampicillin (Carl Roth GmbH, Karlsruhe, Germany) or 100 g/ml streptomycin (Merck)