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Description

The second step in the synthesis of GSH is the addition of the glycine residue to GluCys by the enzyme glutathione synthase (GS)

Following treatment, the medium was replaced with fresh medium containing 0.5 mg/mL of MTT solution, and the plates were incubated for an additional three hours at 37C

NAC is considered more resistant to oxidation compared to cysteine, offering ease of formulation as a solution without the repulsive odor

Similar to the HEK293 ATP6AP1 KO cells, U2OS ATP6AP1 KO displayed increased m compared to control cells, which remained unaffected upon ACT treatment (Fig