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This is used to label usually 23 samples, but up to 5 samples (5-plex) can be labeled and pooled in a single experiment if other formaldehyde/cyanoborohydride isotopomers are used (Wu et al., 2014)

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CHAC1 supplies the matrix cysteine pool in the absence of extracellular cystine To determine if CHAC1 has a discernable influence on the pools of mitochondrial cysteine and GSH in the potential support of Fe-S cluster synthesis, we endeavored to monitor the availability of these metabolites in response to cystine starvation (Fig