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If requirement becomes less, this shows the success of vitamin b12 injection for dogs therapy and decision of administering maintenance dose is dependent on results of laboratory tests

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Multi-dose vials are designed for multiple uses, but every puncture must be performed with new sterile equipment and alcohol-sterilised rubber stopper surfaces.

This is in preparation for the mixing water to be added to the vial, to avoid pressure in the vial, that wont allow you to add much water to it