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This study also identified the depletion of the purine metabolite, hypoxanthine, in chronic fatigue patients (according to Canadian Consensus criteria) which may impair ATP production and contribute to the biochemical changes associated with PEM

Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

The insulin-like growth factor 1 receptor (IGF-1R) is a membrane protein with tyrosine kinase activity

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