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A mild, short-lived headache can also occur in the first day or two after injection

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The denatured proteins were then loaded onto either a 10% or 12% SDS-polyacrylamide gel for electrophoresis and later blotted onto polyvinylidene difluoride (PVDF) membranes

Scopa et al., 2023)

Harmonic restraints of 50.0 kcal mol 1 2 were applied to the solutes, including the crystallographic ions, to allow solvent relaxation while preserving the maximum fidelity to the docking pose, and the Andersen temperature coupling scheme 81,82 was used to control and equalize the temperature