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Cells were cultured in vitro in DMEM medium (Gibco, Thermo Fisher Scientific, Waltham, MA, United States), supplemented with 10% (v/v) fetal bovine serum (FBS) (Euroclone, Italy), 1% penicillin (100 U/ml)/streptomycin (100 g/ml) (Euroclone, Italy), and 1% L-glutamine (2 mM) (Euroclone, Italy) at 37C with 5% CO 2

D.FolchJ.OlloquequiJ.et al

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A maximal cutoff was decided at 12 s to minimize tissue damage [60]