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The PCRs were performed with Platinum SYBR Green qPCR Super Mix-UDG (Invitrogen, Germany) in a Rotor-Gene 6000 cycler (Qiagen, Germany)

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Understanding how the microbiome is involved in establishing, maintaining, and reversing obesity has been an active area of research 14,15,16 , and has included studies investigating links between the microbiome and obesity-related comorbidities such as non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH) 17,18

Understanding that distinction is essential when evaluating liposomal glutathione benefits

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