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At 1/20 the normal rescue dose, the hepatocyte curve descends to the 30% level so the survival of the patient is unclear

Researchers are thus focusing on developing inhibitors that target drug-resistant isoforms, enhancing the effectiveness of existing chemotherapeutics

Materials and Methods Cloning, Site-Directed Mutagenesis, Protein Expression, and Purification Sequences coding for PtGSTU19 (Potri.008G174900) and PtGSTU20 (Potri.008G175000) were amplified by PCR from poplar cDNA using specific primers (Supplementary Table S1) and cloned into pET-12a plasmid (Novagen) between Nde I and Bam HI restriction sites