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Hepatic effects of long-term methotrexate use in the treatment of inflammatory bowel disease

Methods TCGA database mining We downloaded the processed TCGA RNA-seq gene expression data for 50 normal and 373 tumor LIHC samples from The Cancer Genome AtlasData Portal ( We log 2 -transformed the normalized read counts (per million reads mapped) for RNA-seq data (all values less than 1 were assigned value 1 before transformation) but carried out no further normalization

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This is particularly important for maintaining glutathione levels, as oxidative stress can deplete glutathione reserves