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Sanger sequencing revealed a germline insertion in NF1 exon 2 (c.165_166insCT), causing a frameshift and premature truncation that likely disrupted NF1 function in patient iPSCs (Fig

The delay in Hgf and Cyclin D1 expression also coincided with differences in the kinetics of ERK1/2 phosphorylation between the two mice genotypes, suggesting that growth factor stimulation required for hepatocyte progression up to G1/S boundary was also altered