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Then they were treated with 100 g/mL cycloheximide for 10 min and collected from plates by scrapping followed by centrifugation at 200 x g for 5 min

Together the peptides accelerate tissue repair through paired mechanisms BPC-157 creates the ideal healing environment while TB500 mobilizes repair cells to the injury site

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The column was equilibrated with five column volumes of buffer A before the CLs were loaded at a flow rate of 2 ml min 1