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The following day, the blot was washed 3 times with 1 TBST for 15 min each at RT and then incubated with horseradish peroxidaseconjugated goat anti-mouse IgG antibody (1:20000, Jackson ImmunoResearch 115-035-003) diluted in 5% non-fat milk for 1 h at RT

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monocytogenes was determined by cellular adhesion and invasion assays and mice virulence tests, and the expression of virulence factors was determined by Western blot