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A total of 20 mg of fresh-frozen liver, kidney, heart, lung, brain, and skeletal muscle tissue was homogenized with 1 mL ice-cold RIPA buffer (Sigma-Aldrich, R0278) with 1 Proteinase inhibitor (Halt Protease and Phosphatase Inhibitor Cocktail, 100, Thermo Fisher Scientific, 78440)

He is also a nonresident senior fellow at the American Enterprise Institute.

Depending on the degree of heterogeneity, summary statistics were calculated using either a fixed-effects model or a random-effects model

doi: 10.1016/j.sleep.2021.10.018