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sgRNA inserts were PCR-amplified and barcoded by PCR, using primers unique to each condition

Supplementation protocol L-Citrulline and GSH (Setria) were obtained from Kyowa Hakko Bio Co., Ltd

Similarly, denosumab modified radiographic progression and decreased structural damage in erosive hand OA in a placebo-controlled, double-blind, randomized phase 2a clinical trial by acting as RANKL inhibitor by preventing the binding of RANKL to its receptor RANK on osteoclast precursors and reducing the expression of NFATc1 and TRAP

doi: 10.1371/journal.pone.0047891 132 ArizaMERivaillerPGlaserRChenMWilliamsMV

however, its activity is positively correlated with the advanced invasion and metastasis phenotypes of breast cancer in vivo [18]