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Leitao MM Jr, Kreaden US, Laudone V, Park BJ, Pappou EP, Davis JW, Rice DC , Chang GJ, Rossi EC, Hebert AE, Slee A, Gonen M The RECOURSE Study: Long-term Oncologic Outcomes Associated With Robotically Assisted Minimally Invasive Procedures for Endometrial, Cervical, Colorectal, Lung, or Prostate Cancer: A Systematic Review and Meta-analysis

1 ER-IP approach enables rapid purification of ER from mammalian cells

To execute this, 200 L of the examined samples, previously diluted with PBS at a 1:5 ratio (v/v), were combined with standard solutions ranging from 0 to 100 mol/L alongside 200 L of blank PBS solution

For LCMS analysis, metabolite extracts were analysed by high-resolution mass spectrometry with an Orbitrap Exploris 240 (Thermo) coupled to a Vanquish Flex liquid chromatography system (Thermo)