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Subsequently, the cells were fixed with 4% paraformaldehyde at 16 C for 15 min, permeabilized in phosphate buffered saline (PBS) with 0.1% Triton X-100 for 5 min at 16 C, and incubated with first antibodies at 4 C overnight, followed by incubation with FITC-conjugated secondary antibodies at 16 C for 1 h

For instance, a phase II trial in ulcerative colitis patients was mentioned in abstracts

Functional states of myeloid cells in cancer

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The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells