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Dompe, C., Moncrieff, L., Matys, J., Grzech-Leniak, K., Kocherova, I., Bryja, A., Bruska, M., Dominiak, M., Mozdziak, P., Skiba, T., Shibli, J., Volponi, A

p13) and MYST3-CREBBP rearrangement harbors a distinctive microRNA signature targeting RET proto-oncogene

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NAD+ BENEFITS Selective NNMT inhibition substrate-site targeting with minimal off-target activity in preclinical models NAD+ salvage modulation studied for increasing intracellular NAD+ and SAM availability Adipose metabolism investigated for suppression of adipocyte lipogenesis in 3T3-L1 models Small molecule distinct mechanism from peptide-based metabolic compounds (GLP-1 agonists, etc.) Defined pharmacology IC of 1.2 M confirmed in published enzymatic assays WHAT RESEARCHERS LOOK AT NNMT enzyme inhibition kinetics and SAM/1-MNA substrate balance NAD+ and SAM level changes in adipocyte and hepatocyte cell models Lipogenesis suppression in 3T3-L1 differentiated adipocyte assays Polyamine flux enzyme upregulation (ODC, SSAT) and histone methylation In vivo efficacy in diet-induced obesity (DIO) murine models

CAR-T cells have been successfully utilized to effectively treat hematological malignancies, yielding favorable outcomes (Gill and Brudno, 2021)