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These chemical scaffolds frequently yield screening hits due to nonspecific interfering mechanisms such as redox cycling that can generate reactive oxygen species or covalent adducts and metal chelation that can disrupt metalloenzyme function, leading to false-positive results across diverse assay formats (Xie et al., 2011)

Citraconate (20 mM) was used as a substrate

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A typical ghk-cu before and after timeline for a supervised injectable protocol involves a 3-month active cycle followed by a 1-month break