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Description

Replace roller after the recommended number of applications per manufacturer guidelines

Whisker pads were isolated from 5-week-old female C57BL/6 mice as described and were shortly immersed in 70% ethanol in PBS, followed by incubation in Williams E medium containing 400 U/ml penicillin, 400 g/ml streptomycin, and 1 g/ml Fungizone (Life Technologies, Rockville, Maryland, USA) for 10 minutes