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3, 4, respectively)

The increased fibroblasts and keratinocyte proliferation help produce more collagen and form the extracellular matrix needed for tissue repair

Membranes were blocked for 1 hr in blocking buffer (5% w/v solution of non-fat dry milk in TBS-T: 1X Tris-buffered saline (20 mM Tris, 150 mM NaCl) supplemented with 0.1% Tween-20 detergent)