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This regulation covers aflatoxins (AFB1, AFB2, AFG1, AFG2, and AFM1), citrinin (CIT), deoxynivalenol (DON), ergot sclerotia and ergot alkaloids, fumonisins (FB1 and FB2), HT-2 and T-2 toxins, ochratoxin A (OTA), patulin, and zearalenone (ZEN) (European Commission 2023)

The samples underwent denaturation (10 min at 95C), amplification (40 cycles for 15 s at 95C and 1 min at 60C), quantification using a melting curve program (60 - 99C) with a temperature rate of 0.1C/s, and routine monitoring of fluorescence)

44 Lastly, pyroglutamic, a glutathione usage deficiency marker, was elevated in the patients exposed to mold versus our control samples