The following primary antibodies diluted in TBST (containing 4% gelatin and 3% BSA) were used: anti-DRP1 (#8570, 1:3000, Cell Signaling Technology), anti-p-DRP1 S616 (#3455S, 1:3000, Cell Signaling Technology), anti-p-DRP1 S637 (#4867S, 1:3000, Cell Signaling Technology), anti- GAPDH (#60004-1-Ig, 1:5000, Proteintech), anti-Cyclin B1 (#4138, 1:3000, Cell Signaling Technology), anti-p16 (#1002202-23, 1:2000, abcam), anti-p62 (#ab109012, 1:3000, abcam), anti-LC3B (#ab192890, 1:3000, abcam), anti-Pink1 (#23274-1-AP, 1:3000, Proteintech), anti-Beclin1 (11306-1-AP, 1:3000, Proteintech), anti-TFR2 (#A9845, 1:5000, ABclonal), anti-FTH1 (#A19544, 1:5000, ABclonal), anti-HO-1 (#10701-AP, 1:2000, Proteintech), anti-GPX4 (ab125066, 1:5000, Abcam), Anti-HSP60 (66041-1-Ig, 1:3000, Proteintech), anti-SLC7A11 (#26864-1-AP,1:3000, Proteintech), anti-SLC3A2 (#15193-1-AP, 1:3000, Proteintech), anti-NRF2 (#16396-1-AP, 1:3000, Proteintech), anti-BNIP3 (#68091-1-Ig, 1:3000, Proteintech) and anti-Parkin (#2132, 1:3000, Cell Signaling Technology)
PMID: 41842655 Lung Disulfiram Alleviates MTX-Induced Pulmonary Fibrosis by Inhibiting EMT in Type 2 Alveolar Epithelial Cells
c) Antioxidant Activity: BPC-157 exhibits potent antioxidant properties, protecting cells from oxidative damage caused by free radicals
Theres no single dose, as its highly dependent on individual sensitivity
Other Fat-Reduction Methods When youre exploring ways to sculpt your body and eliminate stubborn fat, the options can feel overwhelming
