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After this procedure, presumptive zygotes were controlled and transferred to in vitro culture medium (BO-IVC, IVF Bioscience) covered with mineral oil (BO-Oil, IVF Bioscience) and incubated for a period of 78 days at 38.5 C, 6% CO, 6% O, 88% N, and in a humid environment

Wu J, Xiao H, Sun H, Zou L, Zhu LQ

Explaining the gender reversal from male preponderance in COVID-19 to female in LC is challenging and covering the range of symptoms intimidating

For each sample, 200 l of supernatant was added to 600 l of thiobarbituric acid (TBA) solution, reconstituted in glacial acetic acid, and then incubated at 95 C for 60 min in a dry-block thermostat (Bio TDB-100, Biosan, Latvia) and finally cooled immediately on ice for 10 min