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Based on our functional data, we propose a model where the R-domain transitions from unbound/disordered to bound/ordered upon phosphorylation, residing either between the NBDs when dephosphorylated 35 or near NBD1 but loosely associated

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In most cases, freshly boiled extracts were processed further as described below or, in some cases, lysates were stored frozen at 30 C

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The use of controls without primary antibodies further demonstrated that the staining of vascular and hepatic cell markers was indeed specific (Fig