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Moreover, although the unmethylated CpGs of lipoplex systems are masked preventing an innate immune response, once they are in the general circulation, lipoplex systems may be, similarly like polyplex systems, opsonized by blood proteins (C3, IgG, lipoproteins and fibronectin) resulting in inflammatory reactions (mediated by TNF-alpha, IL-6 and IL- 12) in lungs and liver

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In HeLa cells, the main mechanism of RVI was shown to be activation of HICC (Wehner et al., 2003b), the molecular correlate of which is TRPM2-C plus CD38 (Numata et al., 2012)