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For the experiments requiring medium adaptation, RPMI 1640 medium without L-glutamine (Life Technologies, Cat#: 21870076) was used and supplemented with L-glutamine (Thermo Fisher Scientific, Waltham, MA, Cat#: A2916801) to achieve the desired concentrations

Based on the current studies, these relationships can be categorized into three confidence levels to clearly distinguish between validated, indirectly supported, and speculative associations (Figure 3)

In contrast, a sedentary lifestyle is associated with increased levels of oxidative stress

doi: 10.1016/j.chemolab.2010.04.016 15 GoelA.SheoranI

Neurobiol Stress Microglial SIRT6 confers protection against neuroinflammation-associated depression through NRF2-HO1 signaling