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X-ray crystallography established the first three-dimensional structure of the ghrelin receptor in an inactive conformation using its high-affinity antagonist Compound 21 and a ghrelin receptor-specific antibody in 2020 (Figure 2A) (Shiimura et al., 2020)

4.13.2 Mechanical stress Mechanical testing demonstrates increased tissue stiffness that limits adipocyte expansion and triggers mechanotransduction through focal adhesion complexes (327, 328)