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Biochemistry 52, 57605769 10.1021/bi400405p [DOI] [PMC free article] [PubMed] [Google Scholar] Bartkowiak A., Grzelinska E., Bartosz G

Engineering a pH/glutathione-responsive tea polyphenol nanodevice as an apoptosis/ferroptosis-inducing agent

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Proteolytic activity assay To determine the activity of the proteases against Azocoll and Elastin Congo Red, 2.5 g of each KLIKK protease was diluted in assay buffer (50 mM Tris, pH 8.0, 2.5 mM CaCl 2 , and 0.02% NaN 3 ) to a final volume of 125 l, and then mixed with 125 l of substrate in assay buffer (15 mg ml 1 ), followed by incubation for 2 h (Azocoll) or 16 h (Elastin Congo Red) at 37C with shaking