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After blocking the membrane in 5% skim milk, primary antibody was incubated over night at 4 C

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The evidence that hypotaurine, through its binding to soluble A, reduces the production and aggregation of oligomers, as well as its ability to lessen the toxicity of -amyloid (A) in primary cultured neurons and amyloid plaques in a mouse model, provides support for this assertion (129)

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Changes in circulating histamine were examined by mass spectrometry

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