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After sorting with anti-CD45 magnetic beads, the tumor cells were stained with anti-CD3, anti-CD8, anti-CD4, anti-CD25, anti-CD127, anti-PD-1, anti-TIM-3, and anti-Granzyme B antibodies for 30 min, fixed and permeabilized using a Foxp3/Transcription Factor Staining Buffer Set (eBioscience), then stained with anti-IFN- antibody for 30 min

While most people can meet their magnesium needs through food sources alone, supplements may be necessary in some cases, particularly if youre deficient in the mineral

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Proceedings of the National Academy of Sciences , 106 (5), 15721577

Cell Stem Cell 10 , 556569 (2012)