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Purification from nuclear extracts was performed according to Nakatanis protocol with some modifications 49

The microalga supplementation also increased MIF which indicates that it is a potent antioxidant agent since the gene MIF encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation which plays roles in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoidsa mechanism substantial for protection against environmental triggered stress at the cellular level [31]

Oxidative stress refers to an overabundance of pro-oxidative factors and reactive oxygen species (ROS) compared to antioxidants

The results of brain lesion grade were analysed by Kruskal-Wallis test with Dunns multiple comparisons test