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The GPX4 enzyme, or phospholipid hydroperoxide GPX (PHGPX), is an oxidoreductase that belongs to a family of eight GPXs [44]

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After 45 h of incubation at 25 C, the animals were collected and washed thrice with M9 buffer to remove bacteria and debris

Given that isotope tracer studies show BB is clearly formed following oral l-carnitine ingestion, and similarly, isotope-labeled BB was readily converted into isotope-labeled TMA/TMAO following oral ingestion, we hypothesized that low overall plasma concentrations of gut microbiotaproduced BB in humans may be the result of microbial BB breakdown into TMA, predominantly occurring in the lower intestinal track (colon) distal to the absorption of most nutrients like l-carnitine and BB