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Differentially expressed genes exhibiting two-fold changes and Benjamini and Hochberg-adjusted P values 0.05 were selected

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For each biomarker (e.g., Chlamydia pneumoniae , NLRP3, caspase-1, ASC, NGSDMD, vimentin), single-channel 20 images (covering ~150 10 3 m 2 area/per image) were converted to grayscale and standardized to baseline intensity by using a histogram-based threshold in ImageJ2/Fiji