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10.1073/pnas.71.10.3879 Proc

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Reduced and alkylated peptides were then desalted and concentrated using Chromabond C18WP spin columns (730522, Macherey-Nagel) according to manufacturer protocols

28 When compared against a contemporary education and exercise (EDX) approach in the LEAP randomised clinical trial, EDX was significantly more successful than CSI in both the short and long term, as measured with a global rating of change score