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Production Methods of Glutathione Microbial fermentation method Modern industrial production of glutathione primarily relies on microbial fermentation using engineered strains of yeast or bacteria that overexpress enzymes in the glutathione biosynthesis pathway

Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]

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