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Insertional mutagenesis to inactivate bsaQ a structural component of the secretion apparatus resulted in delayed escape of the bacterium at 6 hours post-infection with the bsaQ mutant exhibiting greater co-localisation with LAMP-1-positive phagosomes compared to the wild-type [143]

Chemical Research in Toxicology 2004

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Peptide cost calculations reveal that storage-related degradation represents a significant expense category

Then, 3.5 l of complex was applied, incubated for 5 s and blotted with a blot force of 2 and blot time of 2 s using a Vitrobot Mark IV (4 C, 95% humidity) and plunge-frozen in liquid ethane