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For intracellular cytokine staining, cells were incubated with Brefeldin A (1:1000, #00-4506-51, Invitrogen, USA), Monensin (1:1000, #00-4505-51, Invitrogen, USA), PMA (10 ng/mL, #P8139, Sigma, USA) and ionomycin (500 ng/mL, #FMS-FZ208, Fcmacs, Nanjing, China) at 37 C for 3.5 h

Higher levels of post-translationally modified p--Syn (p-S) were also detected in the CSF of PD patients than that of controls (Majbour et al., 2016b)

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Abumrad NA, Cabodevilla AG, Samovski D, Pietka T, Basu D, Goldberg IJ