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Specifically, a stringent selection based on variable importance in projection (VIP), fold change (FC), and statistical significance unveiled 14 significantly upregulated metabolites (Figure 5C), with patterns further explored in heatmaps and chord diagrams

Kang, C., Zhang, Y., Zhu, X., Liu, K., Wang, X., Chen, M., Wang, J., Chen, H., Hui, S., & Huang, L

Considering how common TMJ pain is in EDS, this is a noteworthy application of prolotherapy

PMID: 10717044

The PCR products were cleaned up using the ChargeSwitch PCR Clean-Up Kit, pooled in equimolar amounts, and the 187 bp amplicon was gel purified using a 2% E-Gel EX Agarose Gels (ThermoFisher Scientific G401002) and QIAquick Gel Extraction Kit (Qiagen 28704)