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Some longer-term (i.e., when steroids are taken for more than six to 12 months) side effects include diabetes mellitus (poor control of sugar) and osteoporosis (thinning of the bones)

van Ierssel, J., Osmond, M., Hamid, J., Sampson, M., & Zemek, R

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Mito-Nuclear Communication by Mitochondrial Metabolites and Its Regulation by B-Vitamins

5.6 Plasmid purification Following the manufacturers instructions from the PureLink HiPure Expi Plasmid Gigaprep Kit (Thermo Fisher Scientific), bacterial pellets were processed with resuspension, lysis, and precipitation buffers