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Briefly, individual mice were administered 15 g each of RNAi agent with In vivo-jet PEI (Polypulus Transfection Inc, New York, NY) (resulting in a calculated 1:6 charge ratio of nucleic acid backbone phosphates to cationic lipid nitrogen atoms) in a volume of 25 l on day 1 using an endotracheally intserted MicroSprayer aerosoliser (IA-1C

After EGCG pretreatment, endothelial cells could induce the expression of membrane-type-1 matrix metalloproteinase (MT1-MMP), which promoted endothelial cell migration, and Cav-1, which caused tube formation, was significantly decreased, suggesting that EGCG inhibits angiogenesis (131)