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After being stained by hematoxylin (HY-N0116, MedChemExpress, USA) and eosin (HY-D0505A, MedChemExpress, USA), the slices were sealed with neutral balsam (N861409, Macklin, China) and subjected to observation via an optical microscope (CX31-LV320, Olympus, Tokyo, Japan) under 100 magnification